DNA条形码技术用于进口货物中肩突硬蜱的鉴定

江莉1，龚劲聪1，胡龙飞2，匡维华3，杨少团1，曹秀娟1

　　1.南海出入境检验检疫局，广东 佛山 528200；2.深圳出入境检验检疫局；

　　3.湛江出入境检验检疫局

　　摘要：目的 利用DNA条形码技术对入境货物中截获未知种类的蜱进行种属鉴定。方法抽取蜱DNA，用特异性扩增线粒体细胞色素C氧化酶第Ⅰ亚基（COⅠ）基因片段，并测序。利用NCBI进行BLAST比对。结果蜱样本COⅠ基因序列与肩突硬蜱（Ixodes scapularisSay，1821）COⅠ基因匹配度达100%。形态学上，该蜱符合肩突硬蜱的形态学特征。该蜱种在我国尚无检出报道。结论该蜱为肩突硬蜱。这是国内首次报道从进口货物中截获肩突硬蜱。

　　关键词：DNA条形码；肩突硬蜱；国境口岸

　　中图分类号：R184.39　文献标识码：B

　　Application of DNA barcode technology for detection of

　　Ixodes scapularis from imported goods

　　JIANG Li*, GONG Jin-cong, HU Long-fei, KUANG Wei-hua, YANGShao-tuan, CAO Xiu-juan

　　*Nanhai Entry-Exit Inspection and Quarantine Bureau, Foshan,Guangdong 528200, China

　　Abstract:   Objective   To indentify unknown species ofticks intercepted from imported goods.   Methods   Ticks’DNA was extracted and specifically mitochondrial cytochromeC-specific oxidase Ⅰ(ⅠCOⅠ) gene fragment was used and sequenced.The gene sequence was obtained by PCR sequencing and uploaded toNational Center for Biotechnology Information (NCBI) for BLASTanalysis. .  Results   The COⅠgene sequence of ticksample was 100% identical to COⅠgene sequence of Ixodes scapularis(Ixodes scapularis Say, 1821). The morphological characteristics ofthe tick perfectly match that of Ixodes scapularis. There was noreport of the tick species domestically so far.   Conclusion  Here we report the successful application of DNA barcodetechnique for the identification of the first domesticallyintercepted Ixodes scapularis from imported goods.

　　Key words:   DNA barcoding; Ixodes scapularis; Frontierports

　　《中国国境卫生检疫杂志》2014年2月刊