环介导等温扩增技术及其在传染性疾病检测中的应用

罗力涵，张波

　　沈阳国际旅行卫生保健中心，辽宁 沈阳 110016

　　摘要：环介导等温扩增技术（Loop-mediated isothermal amplification method,LAMP）是近年来发展起来的新型恒温核酸扩增技术，与传统的聚合酶链反应（polymerase chainreaction，PCR）相比具有简便、快速、灵敏度高、特异性强等优点，已被广泛应用于传染性疾病的检测。本文就LAMP技术的原理、特点及其在常见传染性疾病检测中的应用进行综述。

　　关键词：环介导等温扩增（LAMP）；传染性疾病；检测

　　中图分类号：R392.1　文献标识码：B

　　Loop-mediated isothermal amplification method for

　　the detection of infectious diseases

　　LUO Li-han, ZHANG Bo

　　Shenyang International Travel Health Care Center, Shenyang,Liaoning 110016, China

　　Abstract:   As a novel method of nucleic acid amplificationdeveloped in recent years, loop-mediated isothermal amplification(LAMP) is more convenient, rapider, more sensitive and specificthan conventional polymerase chain reaction (PCR), and has beenwidely applie to the detection of infectious diseases. This papersummaried the principle, characteristic and applications of LAMPmethod in the detection of infectious diseases.

　　Key words:   Loop-mediated isothermal amplification (LAMP);Infectious diseases; Detection

　　《中国国境卫生检疫杂志》2014年2月刊