环介导等温扩增技术研究进展

孙菲1，鲁少平2，成英2，宋艳辉3

　　1.湖南国际旅行卫生保健中心，湖南 长沙 410007；2.湖南出入境检验检疫局；

　　3.苏州大学附属第一医院

　　摘要：环介导等温扩增技术（LAMP）是一种核酸扩增技术，该方法最大的优点是能够在63℃～65℃的等温条件下扩增特定DNA序列，并在30～60min内可以观察到结果。LAMP已经成功用于许多病毒的检测，本文主要概述与LAMP技术相关的最新发展现状，以及国外应用该技术检测食物致病菌、动物病毒、临床微生物等方面进展。LAMP作为一种快速、简便、灵敏、特异，无需贵重检测设备而易普及的检测技术，在一些基层实验室、流行病学调查和检验检疫等领域具有广阔的应用前景。

　　关键词：环节异等温扩增技术；快速检测；链置换反应；研究进展

　　中图分类号：R34　文献标识码：B

　　Research progress of loop-mediated isothermal amplificationtechnology

　　SUN Fei, LU Shao-ping, CHENG Ying, SONG Yan-hui

　　Hunan International Travel Health Care Center, Changsha, Hunan410007, China

　　Abstract:   Loop-mediated isothermal amplification (LAMP)was a nucleic acid amplification techniques. The biggest advantagesof this method is able to amplify specific DNA sequences under thecondition of 63℃~65℃ isothermal temperature, and the results can beobserved within 30~60 minutes. LAMP has been successfully used inthe detection of many viruses. In this article, we summarized thelatest development of LAMP technology and the research progressesin the application of testing food pathogenic bacteria, animalvirus and microbiology and so on. As a rapid, simple, sensitive andspecific testing techonlogy without expensive equipment, LAMP has abroad applications in some of the basic laboratory, fields ofepidemiological investigation and inspection and quarantineetc.

　　Key words:   LAMP； Fast testing； Strand exchange reaction；Research progress

　　《中国国境卫生检疫杂志》2013年12月刊