DNA条形码技术用于原木携带蚊种的鉴定

　　王颖1，王勇2，高涛3，刘明杰1，李西标1，岳巧云4

　　1.烟台出入境检验检疫局技术中心，山东 烟台 264000；2.临沂出入境检验检疫局；

　　3.山东出入境检验检疫局；4.中山出入境检验检疫局

　　摘要：目的 对临沂口岸进境原木上截获的蚊种进行DNA条形码研究确定蚊种。方法使用双翅目COI基因国际通用引物对原木携带蚊种COI基因进行PCR扩增和序列测定，序列在NCBI网站上进行Blast，确定蚊种。结果待测蚊种COI序列与GenBank中编号为HQ398903.1的骚扰阿蚊（Armigeressubalbatus）序列相似性为99%。结论基于COI基因的DNA条形码技术可成功地对媒介蚊类进行分类鉴定，该技术可弥补蚊虫形态缺失和形态学鉴定知识不足对蚊类鉴定的影响。

　　关键词：DNA条形码；COI基因；蚊

　　中图分类号：R184.31　文献标识码：B

　　Application of DNA barcoding technique for mosquito ofclassification in logs

　　WANG Ying*, WANG Yong, GAO Tao, LIU Ming-jie, LI Xi-biao, YUEQiao-yun

　　*Yantai Entry-Exit Inspection and Quaranine Bureau, YantaiSandong 264000,China

　　Abstract:   Objective   To applicate DNAbarcoding technique which based on COI gene for mosquito ofclassification in logs at Linyi Port.  Methods   Theinternational COI primers of Diptera was used in the test, andmosquito in logs was analyzed by PCR. The amplified fragment wasalso sequenced and blast in the NCBI website.  Results   Sequence of the COI fragment from the unkownwere 99% identical to the published sequence of Armigeressubalbatus numbered HQ398903.1.   Conclusion  DNA barcoding technique based on COI gene could be used toclassification and identification of mosquitoes successfully, andthis method can remedy the deficient shape characteristic and theshortcoming of knowledge of traditional systematicclassification.

　　Key words:   DNA barcoding; COI gene; Mosquito

　　《中国国境卫生检疫杂志》2013年8月刊