上海浦东机场口岸媒介新病原菌的分离鉴定

　　曹晓梅1，陈拓1，祁军2，姚李四1，张晓龙1，郭天宇1，邓耀华3

　　1.中国检验检疫科学研究院，北京 100123；2.天津出入境检验检疫局；

　　3.上海出入境检验检疫局

　　摘要：目的 对上海浦东机场蝇类携带新病原菌进行分离鉴定。方法将上海浦东机场口岸捕获的蝇体细菌分离培养至单克隆，使用Vitek2 Compact进行初步鉴定，扩增16SrRNA基因，进行序列比对。使用Mega5，Neighbour-joining方法，构建分子系统发育树。结果从大头金蝇体表分离出一株菌，疑似造成暴发性脓毒症的污蝇解壳杆菌（Wohlfahrtiimonaschitiniclastica）。进化树分析表明为同种菌，16S rRNA相似性为98.66%。结论 从大头金蝇体表成功分离到W.chitiniclastica菌的新记录。

　　关键词：蝇媒；病原菌；鉴定；系统发育

　　中图分类号：R183.5　文献标识码：B

　　Isolation of a new vector-borne pathogenic bacteria and

　　molecular identification

　　CAO Xiao-mei*, CHEN Tuo, QI Jun, YAO Li-si, ZHANG Xiao-long,GUO Tian-yu, DENG Yao-hua

　　Chinese Academy of Inspection and Quarantine Science Research,Beijing, 100123,China

　　Abstract:   Objective   To identificationa new fly-borne pathogenic bacteria.  Methods   In vitro and in vivo bacteria of fly werecultured respectively.  Monoclonal strain was identified byVitek2 Compact firstly and then 16S rRNA sequence was amplified andanalyzed. Phylogenetic tree was constructed by Mega5 software,Neighbour-joining method.  Results   A bacteria wasisolated from Chrysomyia megacephala, its 16S rRNA sequence sharedsimilarity of 98.86% with Wohlfahrtiimonas chitiniclastica, whichcaused fulminant sepsis in homeless patients. The phylogeneticanalysis indicated that the strain was the same species withWohlfahrtiimonas chitiniclastica.  Conclusion   A new record of W. chitiniclastica strainwas isolated from Chrysomyia megacephala collected from theport.

　　Key words:   Flies-borne; Pathogenic bacteria;Identification; Phylogenetic

　　《中国国境卫生检疫杂志》2013年8月刊