深圳口岸首例入境人员HIV-1毒株近似全基因序列的测定与分析

   刘胜牙1，朱玉兰1，甄胜西1，古莉冰1，刘春芳1，李微1，董瑞玲1，刁慕言1，张树平1，陈琳2

    1.深圳国际旅行卫生保健中心，广东 深圳 518033；2.深圳市疾病预防控制中心

    摘要：目的 获得深圳口岸首例入境人员HIV-1亚型毒株全长基因组序列并分析其特征。方法以深圳出入境检验检疫局HIV确证实验室确证的1例HIV感染者为研究对象，提取病毒RNA，利用逆转录巢式RT-PCR技术，分两段扩增病毒近似全长基因序列，对PCR产物进行核苷酸序列测定，应用生物信息学软件对全基因序列进行系统进化分析。结果获得了8 831 bp长度的近全长基因组序列，系统进化树分析结果显示该毒株与HIV-1CRF01_AE亚型参考株成簇，Bootstrap值为100%，通过与世界上不同国家和地区流行的HIV-1CRF01_AE亚型毒株比较，该毒株与泰国流行毒株的关系最近。结论该毒株是由境外传入的，应对入境人员HIV感染毒株的来源和变异进行密切监测。

    关键词：艾滋病病毒；全基因；出入境人员；基因序列；分析；口岸

    中图分类号：R512.91　文献标识码：B

    Sequencing and analysis of the full-lengthgenomic characterization of

    the fist HIV-1 virus strain among entrypersonnel at Shenzhen port

    LIU Sheng-ya*, ZHU Yu-lan, ZHEN Sheng-xi, GULi-bing, LIU Chun-fang, LI Wei, DONG Rui-ling,

    DIAO Mu-yan, ZHANG Shu-ping, CHEN Lin

    *Shenzhen International Travel Health CareCenter, Shenzhen, Guangdong 518033,China

    Abstract:   Objective  To gain the full-length genome sequence of the first HIV-1 virusstrain among entry personnel at Shenzhen port, and to analyze itscharacterization.   Methods    Peripherialblood plasma was collected. The viral genomic RNA was extracted andamplified in two halves by using nested RT-PCR. The PCR productswere sequenced after being purified. The sequence was analyzed bybiological infamation software.   Results   Thelength genomic sequence of 8831 bp was acquired. The results ofanalysis of phylogenetic relationship suggested the strain wassubtype CRF01_AE, which was further proved by the phylogenetic treewith a high bootstrap vlue (100%). In the phylogenetic tree, theisolated strain was group into clusters with those from. Conclusion   The strain was introduced from abroad. It isnecessary to  enhanced survillance for HIV-1 among entry-enxitpersonnel.

    Key words:   HIV; Full-length gene;Entry-enxit personnel; Genome sequence; Analyze; Port

    《中国国境卫生检疫杂志》2013年6月刊