成都双流国际机场蝇类携带致病肠杆菌分离及耐药性检测

    钟玮1，2，杨雨2，刘杨2，赵锋2，黄筱宇2，陈琳2，裴晓方1

    1.四川大学华西公共卫生学院，四川 成都610041；2.四川国际旅行卫生保健中心

    摘要：目的 了解成都双流国际机场口岸蝇类携带致病肠杆菌耐药性及耐药基因携带情况。方法通过布放蝇笼捕捉蝇类，使用添加有头孢菌素（20μg/ml）的麦康凯平板从捕获的蝇类中分离其携带的致病肠杆菌，对分离株进行耐药性实验、超广谱β内酰胺酶（ESBL）测试及耐药基因型测定。结果从2011年4月至2011年9月捕获的蝇类中分离到致病肠杆菌48株，其中大肠杆菌37株，肺炎克雷伯菌6株，铜绿假单胞菌3株，嗜水气单胞菌2株。对分离到的48株肠杆菌进行抗生素耐药性实验，对阿莫西林、替卡西林、头孢噻吩和头孢呋辛耐药，对美罗培南和亚胺培南不耐药。所有菌株均对8种或8种以上的常见抗生素耐药。分离的大肠杆菌（n= 37）和肺炎克雷伯菌（n =6）均产ESBL，对头孢噻肟的耐药率98.1%，对头孢他啶耐药率11.5%。对产ESBL菌株进行耐药基因测试，TEM型为常见型（67.4%），SHV型（16.3%）多见于肺炎克雷伯菌，未发现同时产生ESBL和AmpC的-超广谱层内酰胺酶（SSBLs）菌株。结论从双流国际机场口岸环境内蝇类分离到致病肠杆菌多重耐药，且携带有产ESBL的耐药基因。

    关键词：蝇类；肠杆菌；ESBL；耐药性；基因型

    中图分类号：R184.33 R512.5　文献标识码：B

    Separation and antibiotic resistance testingof pathogenic enterobacteria isolated from flies at ChengduShuangliu Airport

    ZHONG Wei*, YANG Yu, LIU Yang, ZHAO Feng,HUANG Xiao-yu, CHEN Lin, PEI Xiao-fang

    *College of Public Health, SichuanUniversity, Chengdu, Sichuang 610064,China

    Abstract:   Objective  To study antibiotic resistance and resistance genes of pathogenicenterobacteria isolated from flies at Chengdu ShuangliuAirport.   Methods   Flies cage was used tocatch flies, pathogenic enterobacteria was isolated from theseflies with MacConkey agar supplemented with cephalothin (20 μg/ml),test antibiotic resistance, ectended-spectrum β-lactamase(ESBL) andresistant genotypes of these isolated bacteria.  Results   Pathogenic enterobacteria (n=48) were isolatedfrom flies captured in April 2011 to September 2011, includingEscherchia coli (n=37), Klebsiella pneumoniae (n=6), Pseudomonasaeruginosa (n=3), Aeromonas hydriphila (n=2). Resistance test for48 strains, all resistant to amoxicillin, ticarcillin, cephalothinand cefuroxime, no resistant to meropenem and imipenem. All strainswere 8 or more resistant to common antibiotics. Separation ofE.coli (n=37) and Klebsiella pneumoniae (n=6) were produced ESBL,cefotaxime resistance rate was 98.1%, ceftazidime resistance ratewas 11.5%. ESBL producing strains test resistant genotypes,TEM-type (67.4%) was common type, SHV-type (16.3%) was more commonin Klesiella pneumoniae, Super-spectrum β-lactamase (SSBLs) strainsthat produce the ESBL anf AmpC at the same time was not found.Conclusion   The pathogenic enterbacteria isolated fromflies at ShuangLiu ariport were multi-drug resistant and carry ESBLresistant gene.

    Key words:   Fly; Enterobacteria;ESBL; Resistance; Genotype

    《中国国境卫生检疫杂志》2013年6月刊