双试剂连续监测法测定血清丙氨酸氨基转移酶测量不确定度评定方法的研究

   朱晓强1，史咏梅2，杨泽2，谭华2，刘军卫3，朱玉兰4，唐明慧2，叶立青2，莫秋华2

    1.珠海市质量计量监督检测所，广东 珠海 59000；2.珠海出入境检验检疫局;

    3.珠海市人民医院；4.深圳出入境检验检疫局

    摘要：目的探讨临床检验中连续监测法测定中血清丙氨酸氨基转移酶（ALT）测量不确定度的评定方法。方法采用连续监测法在全自动生化分析仪上测定血清样本ALT的含量，据此原理建立数学模型，进行速率法测定血清中ALT的不确定度分析。结果血清中ALT的扩展不确定度U=4.8 U/L，k=2。结论本研究中分析了仪器本身和检测过程的几个不确定度分量，为进一步分析提供了一些思路。

    关键词：临床化学；测量不确定度；全自动生化分析仪；连续监测法

    中图分类号：R446.11　文献标识码：B

    Study on the uncertainty assessmentmeasurement of alanine aminotransferase by double reagentcontinuous monitoring method

    ZHU Xiao-qiang*, SHI Yong-mei, YANG Ze, TANHua, LIU Jun-wei,

    ZHU Yu-lan, TANG Ming-hui, YE Li-qing, MOQui-hua

    *Zhuhai Supervision Testing Institute ofQuality and Metrology, Zhuhai, Guangdong 519000，China

    Abstract:   Objective  To explore the measurement uncertainty evaluation method of serumalanine aminotransferase (ALT) in clinical examination. Methods   The content of ALT in the serum samples wasdetected by automatic biochemical analyzer by continuous monitoringmethod，and mathematical models was established to perform theuncertainty analysis to determine the ALT in serum. Results   The serum ALT of the expanded uncertainty wasU=4.8 U/L，k=2.  Conclusion   Several components ofproper uncertainty of machines and uncertainty of measurementprocess are considered in this research, which can provide someclues for further research.

    Key words:   Clinical chemistry;Measurement uncertainty; Automatic biochemical analyzer; Continuousmonitoring method

    《中国国境卫生检疫杂志》2012年第6期