3种甲型流感快速检测试剂的性能评价

    杨坤宇1，陈慧1，陈毅歆2，张毅1，李庶甘1，郑清炳2，杨剑1，郑燕平1

    1.厦门国际旅行卫生保健中心，福建 厦门361012；2.厦门大学国家传染病诊断试剂与疫苗工程技术研究中心

    摘要：目的 比较3种甲型流感快速检测试剂的灵敏度。方法利用病毒分离培养液和入境人群的鼻咽拭子标本考查试剂盒的检测灵敏度。结果甲型流感抗原快速检测试剂（FluA-DOT）对8份病毒分离培养液的检测灵敏度达到10-2HA，明显高于其他两种检测试剂；FluA-DOT对入境人群鼻咽拭子标本的检出率为91.67%，也远远高于其他两种检测试剂。结论甲型流感抗原快速检测试剂（FluA-DOT）的检测时间短、灵敏度高，适用于口岸现场的甲型流感快速筛查。

    关键词：甲型流感；快速检测；性能评价

    中图分类号：R511.7　文献标识码：B

    Evaluation of three kinds of quick assay kitsfor detecting

    influenza A virus

    YANG Kun-yu*, CHEN Hui, CHEN Yi-xin, ZHANGYi, LI Shu-gan, ZHENG Qing-bing,

    YANG Jian, ZHENG Yan-ping

    *Xiamen International Travel HealthcareCenter, Xiamen, Fujian 361012，China

    Abstract:   Objective  To evaluate the sensitivity of three different kinds of assay kitsfor detecting  influenza A virus.  Methods   Toanalyze the sensitivity of assay kits for influenza A virus byusing the viral culture liquid and nasopharyngeal swabs fromentry-exit travelers as samples.  Results    All the kits were tested against 8different kinds of strains, the FluA-DOT kit displayed asensitivity of 10-2HA, it was much better than other kits. Thesensitivity of FluA-DOT reached 91.67% when the kit was used forthe detection of Nasopharyngeal swab from the entry people, and itwas also better than other kits.  Conclusion   TheFluA-DOT kit need a short detection time and have a goodsensitivity, it is fit for the rapid detection of influenza A virusat frontier ports.

    Key words:   Influenza A virus;Rapid detection; Evaluation

    《中国国境卫生检疫杂志》2012年第5期