3种荧光定量聚合酶链反应试剂筛查入境人员中疑似肺结核病例的效果比较

    马艳艳1，王永亮1，李辉2，石洁2，马晓光2

    1.河南国际旅行卫生保健中心，河南 郑州 450016；2.河南省疾病预防控制中心

    摘要：目的比较3种荧光定量聚合酶链反应(FQ-PCR)试剂检测痰标本中结核杆菌的灵敏度和特异性,探讨FQ-PCR在国境口岸快速检测结核病人的价值。方法　对129例疑似肺结核患者的痰标本分别采用FQ-PCR试剂法、痰涂片抗酸染色法、改良罗氏培养法检测结核分枝杆菌；以培养法为金标准比较艾康、匹基和博奥3种FQ-PCR试剂的特异性和灵敏度。结果　艾康检测疑似肺结核病病例、涂片镜检阳性和涂片镜检阴性者的灵敏度为90.12%、93.55%、78.95%，特异度为95.65%、100%、95.65%；匹基检测疑似肺结核病病例、涂片镜检阳性和涂片镜检阴性者的灵敏度为87.50%、95.16%、61.11%，特异度为100%、100%、100%；博奥检测疑似肺结核病病例、涂片镜检阳性和涂片镜检阴性者的灵敏度为86.59%、95.24%、57.89%，特异度为100%、100%、100%。结论　FQ-PCR是一种简便快速方法，对于涂片镜检阳性结核病病例的检测具有很高的敏感性和特异性，对于涂片镜检阴性结核病病例检测有很高的特异性，适于国境口岸出入境人员中结核病病例的快速检测。

    关键词：结核分枝杆菌；痰标本；痰涂片；培养；荧光定量PCR；荧光定量聚合酶链反应

    中图分类号：R521　文献标识码：B

    Comparison of effects on three kits offluorescence quantitative PCR   examining suspected caseswith tuberculosis at frontier ports

    MA Yan-yan*,  WANG Yong-liang, LI Hui,SHI Jie, MA Xiao-guang

    *Henan International Travel Health CareCenter, Zhengzhou, Henan 450016,China

    Abstract:   Objective  To explore the value of fluorescence quantitative PCR examiningtuberculosis at frontier ports through comparing the sensitivityand specificity of  three kits for detecting mycobacteriumtuberculosis in sputum specimens.  Methods   Thespecimens of sputum from 129 cases with suspected tuberculosispatients were detected by three fluorescence quantitative PCRkits(AIKANG,PIJI,BOAO), acid - fast stain, culture with LowensteinJensen (L - J)medium, respectively, the sensitivity and specificityof three fluorescence quantitative PCR kits were acquired comparingculture results.  Results   For detecting suspectedtuberculosis and smear positive cases and smear negative cases, thesensitivity and specificity  of AIKAN were90.12%,93.55%,78.95% and 95.65%,100%,95.65%, respectively. Thesensitivity and specificity of PIJI were 87.50%, 95.16%,61.11% and100%,100%,100%, respectively. The sensitivity and specificity ofBOAO were 6.59%、95.24%、57.89% and100%、100%、100%,respectively.  Conclusion  Fluorescence quantitative PCR, which is a rapid and sample method,own high specificity and sensitivity for smear positive cases andhigh specificity for smear negative cases, It is a good tool forexamining tuberculosis at frontier ports.

    Key words:   Mycobacteriumtuberculosis; Sputum specimens; Acid-fast stain; Culture;Fluorescence quantitative PCR; FQ-PCR

    《中国国境卫生检疫杂志》2012年第5期