2009-2010年黑龙江中俄边境口岸鼠类动物中汉坦病毒分子流行情况调查及分析

    侯咏1，李大鹏2，刘宇鹏2，鞠文东1，程成1，高丹凝1，富英群1，赵亚双2

    1.黑龙江出入境检验检疫局卫检处，黑龙江 哈尔滨 150090；2.哈尔滨医科大学

    摘要：目的研究黑龙江省中俄边境6个口岸鼠类动物的种类和携带汉坦病毒情况及病毒基因分型，为口岸防控肾综合症出血热提供科学依据。方法采用夹夜法和鼠笼法捕获鼠类，荧光定量PCR（TaqMan探针法）对鼠肺标本进行检测。结果此次调查共捕获鼠类动物295只，经鉴定隶属3科8属10种，其中黑线姬鼠为优势种群，占42.37%。检测出汉坦病毒核酸阳性鼠肺标本22份，鼠总带毒率为7.46%，其中，汉滩型15份，汉城型共7份。在调查的6个口岸中逊克、抚远、黑河、东宁和绥芬河5个口岸发现了汉坦病毒核酸阳性标本。结论本次共调查黑龙江省中俄边境6个口岸，其中有5个口岸发现了汉坦病毒核酸阳性标本，北部边境逊克、抚远和黑河3个口岸均发现了汉坦病毒核酸阳性标本，东部边境东宁、绥芬河、牡丹江3个口岸中东宁和绥芬河发现了汉坦病毒核酸阳性标本，黑龙江省口岸肾综合症出血热防控形势依然严峻。

    关键词：汉坦病毒；鼠类动物；荧光定量PCR；基因分型

    中图分类号：R512.8；R184.38　文献标识码：B

    Epidemiologic investigation and analysis onHantavirus carried by rodents at Heilongjiang frontier portsbetween Sino-Russian border in 2009~2010

    HOU Yong*, LI Da-peng, LIU Yu-peng, JUWen-dong, CHENG Cheng,

    GAO Dan-ning, FU Ying-qun, ZHAO Ya-shuang

    *Heilongjiang Entry-exit Inspection andQuarantine Bureau, Harbin, Heilongjiang 150090, China

    Abstract:   Objective  To investigate the rodent species and Hantavirus infections atHeilongjiang frontier ports between Sino-Russia border, and toprovide scientific evidence for hemorrhagic fever with renalsyndrome prevention.  Methods   Night trappingmethod and mouse cages were used to capture rodents. The rodentlung tissues were detected by real-time fluorescence quantitativePCR.  Results   Two hundred and ninety five capturedrodents were identified as 3 families 8 genera 10 species. Apodmusagrarius was the dominant specie. A total of 22 samples carriedHantavirus with the infection rate of 7.46%. In which, 15 samplesbelonged to HNTV and 7 samples belonged to SEOV. Hantaviruses werecarried by rodents in Xunke, Fuyuan, Heihe, Dongning, Suifenhe ofHeilongjiang province.  Conclusion   Hantaviruseswere carried by rodents at five ports between Sido-Russia border inthis study. The situation of hemorrhagic fever with renal syndromeprevention was still grim.

    Key words:  Hantavirus；Rodents；Real-time fluorescence quantitativePCR；Genotyping

    《中国国境卫生检疫杂志》6月刊