深圳口岸国际旅行人员中HIV-1感染者耐药基因型检测分析

    刘胜牙，朱玉兰，刘春芳，李微，古莉冰，张登峰，俞杨

    深圳国际旅行卫生保健中心，广东 深圳 518033

    摘要：目的对深圳口岸1996—2008年已确诊的68例人类免疫缺陷病毒（HIV-1）感染者进行耐药性突变分析，以了解深圳口岸国际旅行者HIV-1耐药性毒株的流行情况。方法应用巢式RT-PCR扩增HIV-1聚合酶基因，对所获得的PCR产物直接测序，再将所获得的序列与国际耐药数据库比对，辨别耐药性突变位点。结果在68例HIV-1感染者中，经巢式RT-PCR扩增并获得聚合酶基因序列68份。耐药分析结果表明，本研究人群未检测到蛋白酶抑制剂（PI）原发耐药突变；11例感染者发生反转录酶抑制剂（RTI）耐药基因突变，基因突变率为16.17%；其中核苷类和非核苷类反转录酶抑制剂的耐药突变率分别为7.35%（5/68）和8.82%（6/68）。结论本研究分析了深圳口岸国际旅行者HIV-1感染者耐药突变的流行情况，这对于我国的艾滋病预防和控制工作具有重要指导意义。

    关键词：艾滋病毒HIV-1；感染者；基因；耐药突变；国际旅行者；口岸

    中图分类号：R512.91　文献标识码：B

    Analysis on genotypes of HIV-1 drugresistance mutations among international travelers at Shenzhenports

    LIU Sheng-ya, ZHU Yu-lan, LIU Chun-fang, LIWei, GU Li-bing, ZHANG Deng-feng, YU Yang

    Shenzhen International Travel Health Carecenter, Shenzhen, Guangdong 518033,China

    Abstract:   Objective  To understand the epidemiology of HIV-1 drug resistance strains，and to analyze drug resistance associated mutations of HIV-1 among68 plasma samples at Shenzhen ports from 1996 to 2008. Methods   HIV-1 pol gene was amplified by nest RT-PCR，PCR products were directly sequenced. Drug resistance associatedmutations in protease and reverse transcriptase regions wereanalyzed with Standford University HIV/AIDS Drug ResistanceDatabase.    Results    The mutationsconferring secondary resistance to PI were detected in 11 of 68patients, （16.17%）, and none of the isolates had major resistancemutations in the protease gene. The resistance-associated to NRTIand NNRTI in the pol gene were 5（7.35%） and 6（8.82%），respectively.   Conclusion   This study revealsthe emergence of drug resistance associated mutations of HIV-1currently circulating among international travelers. It is believedthat this information may help to guide recommendation forstrategies of AIDS prevention and control in China.

    Key words:   HIV-1； Infection;Genotype; Drug resistance associated mutations； Internationaltravelers; Port

    《中国国境卫生检疫杂志》