一起外轮群体性甲型H1N1流感病例的实验室检测及分析

    周冬根1，孙大为1，张升1，翟敏1，毛宗礽1，郑婧懿1，赵瑞2，戚立松2

    1.宁波国际旅行卫生保健中心，浙江 宁波 315012；2.北仑出入境检验检疫局

    摘要：目的 分析、溯源一起外籍船员群体性流感的原因。方法结合流行病学调查结果，采集所有船员的鼻咽拭子，处理后利用实时荧光PCR法对样本进行甲型流感、乙型流感、禽流感、甲型H3N2流感及新型甲型H1N1流感的检测；同时对所选样本进行RT-PCR确证检测及HA基因测序分析并构建分子进化树。结果实时荧光PCR检测结果显示，6例患者的甲型流感病毒及新型甲型H1N1流感病毒检测均为阳性，但均未检出乙型流感、禽流感及甲型H3N2流感病毒；RT-PCR确证实验以及HA基因测序结果也表明为新型甲型H1N1流感病毒阳性。结论引起本次群体性流感事件的原因为新型甲型H1N1流感病毒感染。分子进化分析表明，引起本次流感事件的病毒株与从香港分离得到的病毒株属同一分支。

    关键词：外轮；群体性病例；流感；检测；甲型H1N1

    中图分类号：R511.7　文献标识码：B

    Laboratory detection and analysis on group ofcases infected with

    influenza A(H1N1) from foreign ship

    ZHOU Dong-gen*, SUN Da-wei, ZHANG Sheng, ZHAIMin, MAO Zong-reng, ZHENG Jing-yi，ZHAO Rui, QI Li-song

    *Ningbo International Travel HealthcareCenter, Ningbo， Zhejiang 315012, China

    Abstract:   Objective  To analysis and trace back the pathogen of group cases infectedwith influenza A (H1N1) from foreign ship. Methods   Considering epidemiological analysis, the nasalswab and throat swab from every infected sailors were collected andthe pathogens as influenza A, influenza B, avian influenza, H3N2and H1N1 were detected with real-time fluorescent PCR. The methodsof RT-PCR and HA gene sequencing analysis were also used toidentify the separated strains.   Results   Theresult of real-time fluorescent PCR showed that all the 6 patientswere positive of H1N1 virus. The positive samples were identifiedto be H1N1 virus with RT-PCR and sequencing.  Conclusion   The pathogen of the group of cases is theinfection of H1N1 virus, and originated from the strain separatedfrom swine infected by H1N1 virus in HongKong.

    Key words:   Foreign ship; Group ofcases; Influenza; Detection; Influenza A(H1N1)

    《中国国境卫生检疫杂志》