海港口岸输入性创伤弧菌分离鉴定与分子分型研究

    胡群 韩辉 雷磊 邹春颖 马研

    1.大榭出入境检验检疫局卫生处，浙江 宁波315812；2.中国检验检疫科学研究院

    摘要：目的 对海港口岸输入性创伤弧菌DXV1108进行鉴定和分子分型研究。方法从一艘越南入境船舶的压舱水中分离获得1株病原菌，采用Vitek 2Compact全自动微生物分析系统进行分类鉴定和抗生素耐药性分析，并采用多位点序列分析（MLST）方法进行分子分型。结果该菌株99%的概率为创伤弧菌，对抗革兰氏阴性菌的抗生素敏感，MLST分析显示为一新发现的序列型。结论本研究对海港口岸防控输入性致病弧菌引发的传染病有重要意义。

    关键词：创伤弧菌；鉴定；分子分型；多位点序列分析；压舱水

    中图分类号：R128　文献标识码：B

    Identification and molecular subtyping ofVibiro Vulnificus

    introduced in ballast water

    HU Qun*, HAN hui, LEI Lei, ZHOU Chun-ying，MAYan

    *Daxie Exit-Entry Inspection and QuarantineBureau, Daxie, Ningbo 315812, China

    Abstract:   Objective  To identify and analyze the molecular subtyping of a VibiroVulnificus strain DXV1108 which introduced from seaport. Methods   A germ straim from ballast water was collectedfrom a vietnam-entry ship, Vitek 2 Compact Fully Automated TestingSystem was adopted to indentify and analyze the vibrio strain’sdrug-resistance and  the method of MLST was used to analyzethe strain’s molecular subtyping.   Results  The germ strain was confirmed as Vibiro Vulnificus (probability99%), it was sensitive to the antibiotics of gram-negative bacteriaand belong to a new sequence type by MLST. Conclusion   This investigation would be of greatsignificance for prevention introduced vibiro infections atseaport.

    Key words:   Vibiro Vulnificus;Identification; Molecular subtyping； MLST; Ballast water

    《中国国境卫生检疫杂志》