两种结核分枝杆菌快速检测方法的比较

    吴健，张建明，何洪涛，陈建东，黄永欣，雷兆良，朱云枫，黄红敏

    广东国际旅行卫生保健中心，广东 广州 510635

    摘要：目的比较荧光定量PCR检测和直接扩增检测两种方法对结核分枝杆菌检测的敏感性、特异性，及在国境口岸卫生检疫工作中的应用前景。方法应用结核分枝杆菌荧光定量PCR和直接扩增检测47份疑似或确诊活动性肺结核空腹痰标本，并与液体培养结果进行比较。结果荧光定量PCR法与直接扩增法对结核分枝杆菌检测敏感性分别为46.7%和100%，特异性分别为81.2%和84.4%。结论结核分枝杆菌直接扩增检测是一种较为敏感而特异的快速检测方法，在国境口岸卫生检疫部门具有重要的应用价值。

    关键词：结核分枝杆菌；液体培养；荧光定量PCR；结核分枝杆菌直接扩增检测

    中图分类号：R183.3　文献标识码：B

    Comparision of two kinds of rapid testmothods for

    mycobacterium tuberculosis

    WU Jian, ZHANG Jian-ming，HE Hong-tao, CHENJian-dong, HUANG Yong-xin,

    LEI Zhao-liang, ZHU Yun-feng， HUANGHong-min

    Guangdong International Travel HealthcareCenter, Guangzhou,Guangdong 510635, China

    Abstract:   Objective  To compare the sensitivity and specificity of two kinds of rapidtest of mycobacterium tuberculosis (fluorescence quantitative PCRand Amplified MTB Direct), and to evaluate their practical value atport.   Methods   The specimens of sputum wereisolated from 47 cases of suspected or diagnosed tuberculosispatients, M.tuberculosis were detected by fluorescence quantitativePCR and Amplified MTB Direct respectively. The results werecompared with that of the liquid culture.  Results   The sensitivity of fluorescence quantitativePCR and Amplified MTB Direct were 46.7% and 100% respectively, andthe specificity were 81.2% and 84.4% respectively. Conclusion   Amplified MTB Direct method is a rapid,sensitive and specific method for the diagnosis of tuberculosis. Ithas a practical value for TB control at port.

    Key words:   MycobacteriumTuberculosis; Liquid culture; Fluorescence quantitative PCR,Amplified M.TB Direct Test

    《中国国境卫生检疫杂志》