ELISA质控存在的问题探讨

    杨寿旺，李睿，周振达，黄建可

    温州国际旅行卫生保健中心，浙江 温州 325000

    摘要：目的 探讨ELISA检测法质量控制方法。方法对目前ELISA质量控制方法及其存在的问题进行详细的分析，寻找其问题存在的原因。结果ELISA质量控制方法理论在实际应用中存在许多问题，其根源在于作为ELISA质控指标的S/CO值是一比值，不具有溯源性。结论S/CO值不适宜作为ELISA质量控制指标。

    关键词：质量控制；酶联免疫吸附试验；样本；吸光度/临界值；质控图；溯源性

    中图分类号：R446.6　文献标识码：B

    The problems in ELISA quality-control

    YANG Shou-wang， LI Rui， ZHOU Zhen-da， HUANGJian-ke

    Wenzhou International Travel Health CareCenter， Wenzhou, Zhejiang 325000，China

    Abstract:   Objective  To study ELISA quality control method.  Methods   The current ELISA quality control method andthe problems were analyzed and the reasons of the problem existingwere found.  Results   There were many problems inpractical application of the current theory of ELISA qualitycontrol method, it roots in S/CO as the ELISA quality control indexwas a ratio value, did not have traceability.  Conclusion   S/CO values are not appropriate for theindex of ELISA quality control.

    Key words:   Quality control;ELISA; Sample; Cut-off absorbance ration; Levey-Jenning;Traceability

    《中国国境卫生检疫杂志》