2010年中蒙边境甘其毛都口岸地区蜱携带病原体调查研究

    杨宇1，张晓龙1，乔舜2，曹晓梅1，王林1，郝广福2，胡健萍1，杨志红1，王静1

    1.中国检验检疫科学研究院卫生检疫研究所，北京100123；2.内蒙古出入境检验检疫局

    摘要：目的为了解中蒙边境蜱传疾病病原体携带情况，对2010年中蒙联合监测小组在内蒙古乌拉特中旗甘其毛都口岸地区采集的162只亚东璃眼蜱进行病原体检测。方法采用RT-PCR法检测蜱标本中的森林脑炎病毒、克里米亚-刚果出血热病毒、布尼亚病毒，PCR方法检测蜱标本中的伯氏疏螺旋体、西伯利亚立克次体、贝氏柯克斯体，嗜吞噬细胞无形体。结果森林脑炎病毒、克里米亚-刚果出血热病毒、布尼亚病毒、嗜吞噬细胞无形体检测结果均为阴性，PCR方法检出5例伯氏疏螺旋体、3例西伯利亚立克次体、16例贝氏柯克斯体，检出率分别为3.7%、2.2%和11.9%，并发现2例伯氏疏螺旋体和贝氏柯克斯体复合感染的情况。结论中蒙边境地区蜱携带伯氏疏螺旋体、贝氏柯克斯体和西伯利亚立克次体等病原体，应有针对性地加强当地蜱类和蜱传疾病的监测和防控。

    关键词:中蒙;口岸；亚东璃眼蜱；蜱传病原体；调查

    中图分类号：R184.39　文献标识码：B

    Investigation on tick-borne pathogens atGanqimaodu port

    between Sino-Mongolia border

    YANG Yu*，ZHANG Xiao-long, QIAO Shun, CAOXiao-mei, WANG Lin,

    HAO Guang-fu，Hu Jian-ping，YANG Zhi-hong, WANGJing

    *Chinese Academy of Inspection and Quaranine,Beijing 100123,China

    Abstract:   Objective  To test for the presence of tick-borne pathogens in 162 adultticks（Hyalomma asiaticum kozlovi）collected at Ganqimaodu portsbetween Sino-Mongolia border in 2010.  Methods  RT-PCR was proceeded to detect tick borne encephalitis virus(TBEV), Crimean-Congo haemorrhagic fever virus (CCHF),Bunyaviruses; PCR was proceeded to detect Borrelia,Rickettsiasibirica,Coxiella burnetii, Anaplasma phagocytophilum from RNA andDNA derived from ticks respectively.  Results  RT-PCR and PCR results of TBEV, CCHF, Bunyaviruses and Anaplasmaphagocytophilum were all negative , Borrelia was identified in 3.7%of ticks(5/162), Rickettsia sibirica was identified in 2.2% ofticks(3/162),Coxiella burnetii was identified in 11.9% ofticks(16/162), Borrelia and Coxiella burnetii were found coexist intwo ticks.   Conclusion   These findings showan active role of ticks in maintaining Borrelia, Rickettsiasibirica,Coxiella burnetii in the borders regions betweenSino-Mongolia, It is necessary to strengthen tick-borne diseasessurveillance in this region.

    Key words:   Sino-Mongolia, Ports,Hyalomma asiaticum kozlovi，Tick-borne pathogens, Investigation

    《中国国境卫生检疫杂志》