蜱类携带粒细胞无形体的核酸检测及核酸序列分析

   宋锋林1，王静2，张晓龙2，高玉峰1，王有1，程晓兰1，姜陆1，吴刚1，薛芳1，刘洪文1

    1.辽宁出入境检验检疫局，辽宁 大连 116001；2.中国检验检疫科学研究院

    摘要：目的 了解蜱类自然感染粒细胞无形体（anaplasmaphagocytophilum）的状况，为人粒细胞无形体病的预防、控制提供技术支持。方法利用分子生物学技术，通过对野外采集的蜱类样品的核酸（DNA）提取、聚合酶链反应（套式PCR）、凝胶电泳分析以及核酸序列分析等，对蜱类携带的粒细胞无形体进行确认。结果在大连地区采集的长角血蜱（Haemaphysalislongicornis）中检测到粒细胞无形体。序列分析结果表明，与GenBank数据库中已注册的粒细胞无形体核酸序列有高度的相似性(97%~99%)。结论我国大连地区存在蜱粒细胞无形体感染。应加强对该地区蜱类的监测和控制，防止因蜱类叮咬导致人粒细胞无形体病的暴发流行。

    关键词：蜱；粒细胞无形体；核酸检测；核酸序列分析

    中图分类号：R184.39　文献标识码：B

    Detection and sequence analysis on nucleicacid of Anaplasma

    Phagocytophilum in ticks

    SONG Feng-lin*, WANG Jing, ZHANG Xiao-long,GAO Yu-feng, WANG You, CHENG Xiao-lan,

    JIANG Lu, WU Gang, XUE Fang, LIU Hong-wen

    *Liaoning Entry-Exit Inspection andQuarantine Bureau, Dalian， Liaoning 116001,China

    Abstract:   Objective  To understand the natural infection of anaplasma phagocytophilum inticks, and to provide the technical support for setting upprevention and control measures to human granulocyticanaplasmosis.   Methods   The DNA extraction ofwild ticks samples, polymerase chain reaction (nested PCR), gelelectrophoresis and gene sequence analysis were conducted toconfirm the types of pathogens in Ticks.  Results  The specific nucleic acid fragments of anaplasma phagocytophilumwere detected in Haemaphysalis longicornis collected from Dalian ofchina. The similarities of the nucleotide sequence of anaplasmaphagocytophilum with those deposited in GenBank were 97%~99%.Conclusion   It is concluded that the infection withanaplasma phagocytophilum is demonstrated for the first time inticks from Dalian of china.

    Key words:   Tick; Anaplasmaphagocytophilum; Nucleic acid detection; Sequence analysis

    《中国国境卫生检疫杂志》