不同保存方法对蚊虫RNA保护效果的评价

    孙立新1，胡红霞2，朱国强1，杨庆贵1

    1.江苏出入境检验检疫局医学媒介生物监测实验室，江苏 南京210001；2.安徽理工大学医学院

    摘要：目的研究适合口岸媒介生物监测的蚊虫样本保存方法，并对蚊虫RNA保护效果进行评价。方法采用普通冰箱、低温冰箱、液氮、RNALater等保存方式，对蚊虫进行1周、1个月、6个月保存，提取蚊虫RNA，比较这些保存方式对蚊虫RNA保护效果。结果RNALater与液氮对蚊样本均具有良好的保存效果，在1周、1个月、6个月内，蚊虫RNA均未见明显降解；低温冰箱在1周、1个月内对蚊样本有较好的保存效果，蚊虫RNA未见明显降解，但6个月后，蚊虫RNA降解明显；常温冰箱（-20℃）保存效果不佳，仅在1周之内对蚊虫核酸有一定的保护效果，1个月后，RNA发生明显降解。结论RNALater、液氮和低温冰箱在1个月内对蚊虫RNA均具有较好的保存效果，但RNALater具有保存时效长、使用方便等优点，更适用口岸蚊虫监测中样本的保存和运输。

    关键词：蚊；样本；核糖核酸；保存；效果；评价

    中图分类号：R184.31　文献标识码：B

    The evaluation of the protective effect ofdifferent

    preservation methods to mosquito RNA

    SUN Li-xin*, HU Hong-xia, ZHU Guo-qiang, YANGQing-gui

    *Jiangsu Entry-exit Inspection and QuarantineBureau, Nanjing, Jiangsu 210001,China

    Abstract:   Objective  Searching for preservation methods of mosquito samples forvector-monitoring at ports,and to evaluate protective effect ofthese preservation methods.   Methods   Theordinary refrigerator, low temperature refrigeration, liquidnitrogen and RNALater preservation methods were used, meanwhile,the mosquitoes were preserved for a week, a month and six months,and then the mosquito RNA was extracted.  Results   The RNALater and nitrogen had the bestprotective effect for mosquito samples, the mosquitoes RNA were noobvious degradation within a week, a month and six months; lowtemperature refrigerator had better protective effect, mosquitoesRNA has no significant degradation in a week and one month, butafter six months, mosquito RNA degradation significantly; theordinary refrigerator (-20 ℃) poorly preserved, it only had aprotective effect for mosquitoes RNA within a week, more than aweek later, RNA was obvious degraded.  Conclusions  RNALater, low-temperature refrigerator and liquid nitrogen all hadbetter protective effect for mosquito samples in one month, butRNALater had advantage of a long time protection effect andconvenient, it is more suitable for mosquito sample preservationand transportation at ports.

    Key words:   Mosquito; Sample; RNA;Preservation; Effect; Evaluation

    《中国国境卫生检疫杂志》