培养条件对海水中菌落总数测定的影响

    谢昭聪1，郑文丽1，徐立俊1，李小燕2，黄明燕1，袁新竹1，杜林3

    1.盐田出入境检验检疫局船舶检验检疫科，广东 深圳518045；2.深圳机场出入境检验检疫局；

    3.仲恺农业工程学院食品质量安全系

    摘要：目的了解平板计数法测定海水中菌落总数时适宜的培养条件：培养基、培养温度和培养时间。方法深圳水域的海水样品在不同培养基、培养温度条件下，经过不同培养时间培养后进行菌落总数计数。结果在培养温度为25℃、30℃、36℃条件下，在相同培养时间内，2216E琼脂的菌落总数明显高于营养琼脂的菌落总数及平板计数琼脂的菌落总数（P＜0.05）；采用2216E琼脂进行菌落总数测定，在培养温度30℃培养96h后，菌落总数达到最高，且菌落总数高于25℃和36℃培养时的菌落总数（P＜0.05）。结论海水中菌落总数测定时应注意培养时间的控制。2216E琼脂适合用于海水菌落总数测定。深圳区域海水中菌落总数测定适宜采用2216E琼脂于30℃培养96h。有关国家标准中海水中菌落总数测定方法所规定的培养温度、培养时间应考虑进行修订完善以准确检测水温较高的南方区域海水。

    关键词：海水；菌落总数；培养条件

    中图分类号：R117 文献标识码：B

    Effects of culture condition on aerobic platecount of seawater

    XIE Zhao-cong*, ZHENG Wen-li, XULi-jun,  LI Xiao-yan, HUANG Ming-yan, YUAN Xin-zhu,DU Lin

    *Shenzhen Entry-exit Inspection andQuarantine Bureau， Shenzhen，Guangdong 518045，China

    Abstract:   Objective  To study studied the suitable medium, culture temperature andculture time, and to determine the aerobic plate count ofseawater.   Methods   Samples of seawater fromShenzhen area were cultured in different media, temperature, afterdifferent time colony-forming unit were counted. Results   In culture temperature of 25℃, 30℃, 36℃, withthe same culture time, colonies numbers on 2216E agar wereobviously more than on the nutrient agar and the plate count agar(P < 0.05); using 2216E agar for aerobic plate count intemperature 30℃ reached the highest number of colonies after 96 h,and the numbers of colony are higher than in 25℃ and 36℃(P <0.05). Conclusion   Culture time should be wellcontrolled when the aerobic plate count of seawater was detected.2216E agar is a suitable medium for aerobic plate count of seawaterin Shenzhen area. In order to accurately detect the aerobic platecount of seawater of the South region where water temperature isrelatively high, the necessary amendments to the culturetemperature, culture time of the relevant national standards shouldbe carried out.

    Key words:   Seawater； Aerobicplate count； Culture condition

    《中国国境卫生检疫杂志》