16S rRNA基因序列分析法在输入性蜚蠊携带病原菌检测中的应用研究

    朱临1，孙立新2，胡红霞1，叶松1，朱国强2，杨庆贵2

    1.安徽理工大学医学院，安徽 淮南 232001；2.江苏出入境检验检疫局

    摘要：目的 应用16SrRNA基因序列分析法对江苏口岸输入性蜚蠊携带的细菌进行快速检测。方法用研磨器将蜚蠊研碎，用LB培养基增菌和分离培养，随机挑选细菌单克隆，提取DNA，对16SrRNA基因进行PCR扩增，扩增产物进行序列分析、比对，确定细菌种属。结果 从输入性蜚蠊中共分离鉴定出23种细菌，分属15属。结论输入性蜚蠊携带多种重要致病菌和条件致病菌，应加强口岸蜚蠊类监测和卫生处理，控制相应传染病的发生和流行。

    关键词：输入性；蜚蠊；细菌；16S rRNA基因序列；检测

    中图分类号：R184.3　文献标识码：B

    Application of 16S rRNA gene sequences oncockroach-carried

    pathogens detection

    ZHU Lin*, SUN Li-xin, HU Hong-xia, YE Song,ZHU Guo-qiang, YANG Qing-gui

    *School of Medicine, 'Anhui University ofScience and Technology, Huai'an,'Anhui 232001,China

    Abstract:   Objective  To survey pathogens carried by imported cockroach at Jiangsu portwith the analytical method of 16S rRNA gene sequences.  Methods   Smash the cockroach with a grinder, isolate thebacteria with LB, extract bacteria monoclonal DNA and then amplify16S rRNA with PCR, and sequence analysis and identificationbacteria species.  Results   In the importedCockroaches, a total of 23 species bacteria was isolated andidentified, belonging to 15 genera. Conclusion   Theresults showed that imported cockroach carry a variety ofpathogenic or opportunistic pathogens. it is needed to strengthenthe monitoring on port cockroach, and prevent the occurrence andprevalence of the corresponding diseases.

    Key words:   Importation；Cockroach；Bacteria；16S rRNA；Detection

    《中国国境卫生检疫杂志》