电磁辐射对N9小胶质细胞STAT3核转位以及磷酸化水平的影响

    郝玉通1，杨学森2，陈纯海2，张广斌2，余争平2

    1.重庆国际旅行卫生保健中心，重庆 401147；

    2.解放军第三军医大学

    摘要：目的 研究STAT3信号分子是否参与电磁辐射诱导的小胶质细胞活化。方法Western-blot检测小胶质细胞STAT3蛋白质表达及磷酸化表达变化，凝胶迁移率实验（ElectrophoreticMobility Shift Assay，EMSA）检测小胶质细胞STAT3的DNA结合活性的变化。结果电磁辐射后小胶质细胞STAT3磷酸化增强，STAT3核转位与DNA结合活性在辐照后6 ~24 h增加，并以12 h最为明显。结论STAT3信号分子参与了电磁辐射诱导的小胶质细胞活化过程。

    关键词：电磁辐射；N9小胶质细胞；STAT3信号分子；磷酸化

    中图分类号：R131　文献标识码：B

    Effect of electromagnetic irradiation inducedSTAT3 DNA binding and phosphorylation in N9 microglial cells

    HAO Yu-tong*, YANG Xue-sen， CHEN Chun-hai，ZHANG Guang-bin， YU Zheng-ping

    *Chongqing International travel healthcarecenter, Chongqing 401147, China.

    Abstract:   Objective  To investigate the possible participation of STAT3 signalingmolecule and activation of microglia after electromagneticirradiation.    Methods   Phosphorylation of STAT3 was tested using western blotting. STAT3DNA binding activity was detected by EMSA.  Results    After electromagnetic irradiation, thephosphorylation of STAT3 was detected rapidly post microwaveexposure. A significant increase of STAT3 DNA-binding ability wasnoted from 6h to 24h after exposure, especially at 12h. Conclusion    The STAT3 signaling molecule mayparticipate in the activation of microglia induced byelectromagnetic irradiation.

    Key words:    Electromagneticirradiation; N9 microglial cell; STAT3 Signaling molecule;Phosphorylation

    《中国国境卫生检疫杂志》