HIV质控血清稳定性的关键影响因素研究

    刘建礼 朱红 张绍福

    北京国际旅行卫生保健中心艾滋病室，北京 100013

    摘要：目的 研究温度、冻融、运输等因素对HIV抗体质控血清稳定性的影响。方法将配置的HIV质控血清分别进行如下处理：（1）置于-20℃、4℃、室温、37℃不同时间；（2）反复冻融；（3）模拟运输。之后用ELISA试剂盒检测样本的反应OD值，统计分析处理后与样本参考值的差异。结果质控血清在-20℃可以保存1年，在4℃不超过3周，在室温下不超过1周，在37℃不超过2d是稳定的。反复冻融对检测值绝对影响不大，但统计学差异显著。运输路途时间超过15 d，样品稳定性得不到保证。结论HIV质控血清必须按照要求进行正确的保存、运输和使用。

    关键词：HIV；质控血清；稳定性；影响因素；研究

    中图分类号： R446.61　文献标识码： B

    The key influence factors on the stability ofHIV quality control serum

    LIU Jian-li, ZHU Hong,  ZHANGShao-fu

    Beijing International Travel HealthcareCenter, Beijing 100013, China

    Abstract:   Objective  To assess the influence of several key factors on the stability ofHIV borderline reactive serum for quality control. Methods   The serum was treated in the followingsituation:（1）-20℃、4℃、37℃ or room temperature for different periodof time， （2）freezing and thawing repeatly， （3）mimic transportation.Then the absorbance (OD) of ELISA test was compared with thereference value.  Results   HIV quality controlserum was stable for one year at -20℃, for no more than 3 weeks at4℃, for less than 1 week at room temperature and for no more than 2days at 37℃. Repeat freezing and thawing caused a gentle butstatistically significant decrease of OD value. Exposure for morethan 15 days during transportation can not assure the stability ofthe serum.  Conclusions   HIV quality control serummust be stored, delivered and used according to the aboverequirement.

    Key words:   HIV; Quality controlserum; Stability； Influence factor; Study