福建口岸首次截获输入性登革热病例实验室诊断

    福建口岸首次截获输入性登革热病例实验室诊断

    王宇平 张建庆 许 卿 张建明 黄恩炯 高 博 王光辉 蔡亨忠

    （福建国际旅行卫生保健中心，福州 350001）

    摘要 〔目的〕通过实验室检测发现福建口岸首次截获输入性登革病例，并进行分子追踪溯源。〔方法〕采用实时荧光RT-PCR和RT-PCR检测方法，并对RT-PCR扩增产物进行核苷酸序列分析。〔结果〕从血清样本中检出登革病毒1型核酸，序列分析表明，该病毒株与来自太平洋群岛分离株在系统进化树上最为接近，序列同源性高。〔结论〕福建口岸首次截获输入性登革热病例，病毒株来源于太平洋群岛地区。

    关键词 口岸；输入性；登革热；实验室诊断

    〔中图分类号〕　R512.8　〔文献标识码〕　B

    Laboratory Diagnosis of Imported Dengue FeverCase Firstly Intercepted at Fujian Port   Wang Yuping,Zhang Jianqing, Xu Qing, Zhang Jianming, Huang ’Enjiong, Gao Bo,Wang Guanghui, Cai Hengzhong. Fujian International TravelHealthcare Center, Fuzhou 350001,China.)

    [Abstract]   Objective  To find and confirm imported dengue fever case intercepted atFujian port.  Method   Real time RT-PCR and RT-PCRwere used to detect serum samples, and the sequencanalysis of theRT-PCR amplification product was adopted The phylogenticrelationship of the virus and other DEV-1 were analyzed,respectiuely.  Result   Dengue virus type 1 nucleicacids were positive in serum sample. Phylogenetic analysis showedthat this virus strain was closed to the isolated strains fromPacific islands in phylogenetic tree, With high sequencehomology.  Conclusion   This case was confirmedimported dengue fever case which was firstly intercepted at Fujianport, and the virus strain might be introduced from Pacificislands.

    [Key words]   Port; Imported;Dengue fever; Laboratory diagnosis