发酵乳酸杆菌PCR快速检测方法研究

    发酵乳酸杆菌PCR快速检测方法研究

    刘云国1,2 侯大海3 刘帅帅4 李俊峰5 周福通5 王建广5 杨大伟2

    （1.中粮屯河股份有限公司食品分析检测研究中心，昌吉831100；2.山东出入境检验检疫局，青岛 266002；

    3.广州京诚检测技术有限公司，广州 511400；4.中国海洋大学，青岛266100；5.青岛科技大学，青岛 266042）

    摘要 〔目的〕 建立一种特异、灵敏的发酵乳酸杆菌快速检测方法。〔方法〕根据发酵乳酸杆菌纤维二糖tuf 基因延伸因子基因tu设计一对PCR引物，进行引物的特异性和灵敏度试验。〔结果〕PCR得到了较好的特异性扩增，其他干扰菌都无扩增，灵敏度达1.3 × 104 cfu/ml。〔结论〕该方法能够实现发酵乳酸杆菌的快速检测，具有灵敏度高、特异性好的特点。

    关键词 发酵乳酸杆菌；PCR；快速检测

    〔中图分类号〕　R516.3　〔文献标识码〕　B

    Study on Rapid Detection of LactobacillusFermentam Using PCR Method   Liu Yunguo1,2, HouDahai3，Liu Shuaishuai4, Li Junfeng5, Zhou Futong5, Wang Jianguang5,Yang Dawei2. (1. Food Analysis and Inspection Research Center ofCOFCO TUNHE Co., Ltd, Changji 831100, China; 2.Shandong Entry-ExitInspection and Quarantine Bureau, Qingdao 266002; 3.GuangzhouBeijing Test Technology Co., Ltd, Guangzhou 511400, China; 4.OceanUniversity of China, Qingdao 266100, China; 5.Qingdao University ofScience & Technology, Qingdao 266042, China.)

    [Abstract]   Objective  To establish a rapid, specific and sensitive method for detectionof Lactobacillus fermentam. Methods   According tocellobiose tuf gene elongation factor tu of Lactobacillusfermentam, a pair of PCR primers was designed. The specificity andsensitivity test were carried using PCR detectionmethod.   Results   This method showed a highspecificity, the interfering bacteria were not amplified. Thesensitivity of the method was 1.3×104 cfu/ml.Conclusion   This method is able to achieve rapid testingof Lactobacillus fermentam with a high specificity andsensitivity.

    [Key words]   Lactobacillusfermentam; PCR; Rapid detection