分子生物学技术在蚊虫分类中的应用

    分子生物学技术在蚊虫分类中的应用

    彭淑琼1 刘春晓2 赵纯中2 徐云庆2

    （1.蛇口出入境检验检疫局，深圳 518054；2.深圳国际旅行卫生保健中心，深圳518033）

    摘要由于蚊虫中存在许多由形态相似的隐种组成的种团或复合体，沿用传统的方法对其分类和鉴别较为困难，因此，寻找合适的特征和方法成为新世纪蚊虫分类工作者研究的重点内容之一。分子鉴别是较为活跃的研究领域之一，它以遗传的本质DNA为依据来阐明物种间的差别，从分子水平上鉴别物种，具有客观、准确的特点，在蚊虫分类中广为应用。而应用分子标志研究群体遗传结构是近年来进展最为迅速的领域之一，目前公认mtDNA部分基因序列和微卫星DNA较为理想。

    关键词 分子生物学；蚊虫；分类

    〔中图分类号〕　R184.31　〔文献标识码〕　B

    Application of  Molecular BiologyTechniques in Mosquito Classification  Peng Shuqiong1, LiuChunxiao2, Zhao Chunzhong2, Xu Yunqing2. Shekou Entry-exitInspection and Quarantine Bureau, Shenzhen 518054,China; 2.Shenzhen International Travel Health Care Center, Shenzhen 518033,China.

    [Abstract]  Because there are manymosquitoes implicit from the morphologically similar speciescomposition of the species-group or complex, the traditionalmethods of their classification and identification is difficult,therefore, to find suitable characteristics and methods of the newcentury, mosquitoes in the study classified workers one ofemphasis. Molecular identification is a more active research areas,it is based on the genetic nature of DNA to clarify the differencesbetween species, from the molecular level, identification ofspecies, with an objective and accurate characteristics of themosquito is widely used classification. The application ofmolecular marker studies of population genetic structure is themost rapid progress in recent years, one of the areas currentlyrecognized as part of the mtDNA sequence and microsatellite DNA ismore desirable.

    [Key words]   molecular biology;mosquitoes; Category; Classification