丹东口岸出口食品中金黄色葡萄球菌及肠毒素检测分析

    丹东口岸出口食品中金黄色葡萄球菌及肠毒素检测分析

    高世光 郝陶光

    （丹东出入境检验检疫局，丹东 118000）

    摘要 〔目的〕了解丹东口岸出口食品中金黄色葡萄球菌的污染状况，检测食品中金黄色葡萄球菌携带肠毒素的情况，为口岸加强食品卫生监管提供科学依据。〔方法〕参照标准GB/T4789.10-2008，进行金黄色葡萄球菌检测,同时用全自动荧光酶标免疫测试系统（mini-VIDAS）检测分离菌株携带肠毒素SEA-SEE情况。〔结果〕885份样本中检出金黄色葡萄球菌15株,检出率1.69%；对15份金黄色葡萄球菌肠毒素进行检测，14株呈阳性，阳性率为93.33%。〔结论〕丹东口岸出口食品存在金黄色葡萄球菌污染，并且产肠毒素的金黄色葡萄球菌占很高的比例，对出口食品应加强金黄色葡萄球菌的检测，检出金黄色葡萄球菌的食品一定要按有关规定严格处理。

    关键词 出口；食品;金黄色葡萄球菌;肠毒素;全自动荧光酶标免疫测试系统；检测

    〔中图分类号〕　R155.5　〔文献标识码〕　B

    Analysis on Staphylococcus aureus andEnterotoxin in Exported Food at Dandong Port  Gao Shiguang,Hao Taoguang. Dandong Entry-exit Inspection and Quarantine Bureau,Dandong 118000,China.

    [Abstract]   Objective  To realize the pollution condition of Staphylococcus aureus inexported food, and to detect Staphylococcus aureusenterotoxin.  Method   The Staphylococcus aureuswere detected according to GB4789•10-2008, the mini VIDAS methodwas adpoted to detect Staphylococcus aureusenterotoxin(SEA-SEJ).  Result   In 885 samples,  15strains of Staphylococcus aureus were detected, the positive ratewas 1.69%. Meanwhile, Enterotoxin was detected in 15 strains ofStraphylococcus aureus by mini-VIDAS method, 14 strains waspositive, the rate was 93.33%.  Conclusion   It isnecessary to enhance to detecte Staphylococcus aureus in exportedfood, and  to deal with the positive food rigorously.

    [Key words]   Export; Food;Staphylococcus aureus; Enterotoxin; Mini-VIDAS; Detect